Generator
Part:BBa_K1403003
Designed by: Sylvia Group: iGEM14_Paris_Bettencourt (2014-10-06)
Limonene synthase (LIMS1) expression cassette
This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Verification
Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator
[edit]
Categories
Parameters
None |