Coding
RGE
Part:BBa_K1442102
Designed by: Becky Seeley Group: iGEM14_Warwick (2014-10-07)
Reverse GFP for E.coli
RGE
Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 799 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 763
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Categories
Parameters
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