Generator

Part:BBa_K1403017

Designed by: Sylvia   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 13:10, 12 October 2014 by Ysylvia (Talk | contribs)

Acetaldehyde dehydrogenase (aldB) expression cassette

aldB CDS under constitutive promoter from Anderson's promoter library.

It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter. This plasmid was transformed together with Part:BBa_K1403019 to produce butter smell in E.Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Verification

AldB.png

Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter

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Categories
Parameters
None