Part:BBa_K1513000

A quorum sensing system which can produce auto-inducer (AHL) itself.

Abstract

Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.

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Quorum sensing

Quorum sensing is the regulation of gene expression in response to fluctuations in cell-population density. Quorum sensing bacteria produce and release chemical signal molecules called autoinducers that increase in concentration as a function of cell density. The detection of a minimal threshold stimulatory concentration of an autoinducer leads to an alteration in gene expression. Gram-positive and Gram-negative bacteria use quorum sensing communication circuits to regulate a diverse array of physiological activities. These processes include symbiosis, virulence, competence, conjugation, antibiotic production, motility, sporulation, and biofilm formation. In general, Gram-negative bacteria use acylated homoserine lactones as autoinducers, and Gram-positive bacteria use processed oligo-peptides to communicate. Recent advances in the field indicate that cell-cell communication via autoinducers occurs both within and between bacterial species. Furthermore, there is mounting data suggesting that bacterial autoinducers elicit specific responses from host organisms. Althoughthenatureofthechemicalsignals,thesignalrelaymechanisms,andthetarget genes controlled by bacterial quorum sensing systems differ, in every case the ability to communicate with one another allows bacteria to coordinate the gene expression, and therefore the behavior, of the entire community. Presumably, this process bestows upon bacteriasomeofthequalitiesofhigherorganisms.Theevolutionofquorumsensingsystems in bacteria could, therefore, have been one of the early steps in the development of multicellularity. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1710
  
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  COMPATIBLE WITH RFC[23]
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  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 987