Composite

Part:BBa_J34200:Design

Designed by: ETH Zurich 2006 iGEM team   Group: iGEM06_ETHZ   (2006-08-15)
Revision as of 13:19, 20 October 2006 by Choutkoa (Talk | contribs) (→‎References)


XOR gate, 2x PoPS input, 1x PoPS output


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 15
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    Illegal XbaI site found at 2172
    Illegal SpeI site found at 2064
    Illegal SpeI site found at 4458
    Illegal PstI site found at 1816
    Illegal PstI site found at 4908
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
    Illegal EcoRI site found at 2502
    Illegal EcoRI site found at 4884
    Illegal SpeI site found at 2064
    Illegal SpeI site found at 4458
    Illegal PstI site found at 1816
    Illegal PstI site found at 4908
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
    Illegal EcoRI site found at 2502
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    Illegal BamHI site found at 9
    Illegal XhoI site found at 1965
    Illegal XhoI site found at 4549
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    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
    Illegal EcoRI site found at 2502
    Illegal EcoRI site found at 4884
    Illegal XbaI site found at 27
    Illegal XbaI site found at 2172
    Illegal SpeI site found at 2064
    Illegal SpeI site found at 4458
    Illegal PstI site found at 1816
    Illegal PstI site found at 4908
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
    Illegal EcoRI site found at 2502
    Illegal EcoRI site found at 4884
    Illegal XbaI site found at 27
    Illegal XbaI site found at 2172
    Illegal SpeI site found at 2064
    Illegal SpeI site found at 4458
    Illegal PstI site found at 1816
    Illegal PstI site found at 4908
    Illegal NgoMIV site found at 3796
    Illegal AgeI site found at 583
    Illegal AgeI site found at 1827
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4392
    Illegal SapI.rc site found at 4740


Design Notes

The initial promoters are omitted, so that the light sensing system can accept PoPs from another device, and the promoter on the end produces PoPs from this device

I lambda repressor fused via TEV cleavage site to alpha subunit of RNAP NTD with 1 RBS (strong)

cI lambda repressor fused via TVMV cleavage site to alpha subunit of RNAP NTD with 1 RBS (strong)

Source

We used here two specific sequences for cleavage by TEV and TVMV proteases.

References

Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, Sreedevi Nallamsetty et al., Protein Expression and PuriWcation 38 (2004) 108–115

Controlled Intracellular Processing of Fusion Proteins by TEV Protease, Rachel B. Kapust and David S. Waughn, Protein Expression and Purification 19, 312–318 (2000)