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Device

Part:BBa_K1355004

Designed by: Maria Clara Tavares Astolfi, Luna Barroco de Lacerda   Group: iGEM14_UFAM_Brazil   (2014-10-06)
Revision as of 22:08, 11 October 2014 by Mctastolfi (Talk | contribs)

Mercury ions bioremediating device

The biorremediator device is composed by our “essential biobrick” (BBa_K1355001) + a MerA gene (BBa_K1355000), represented below:

L6.jpg

In presence of mercury, MerR protein will bind to it so MerT, MerP and MerA protein will be expressed and bioremediation will start! MerP and MerT proteins are responsible for transporting mercury from the periplasm to cytoplasm, leading it to mercury ion reductase (MerA)! The beautiful MerA is responsible for reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave the bacteria.

Bioremediaaator.jpg

Figure 1: Mercury Bacter Hg bioremediator (DH5-alpha transformed with BBa_K1355004)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 1160
    Illegal NgoMIV site found at 2397
    Illegal NgoMIV site found at 2459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 579


[edit]
Categories
//function/degradation
Parameters
None