Part:BBa_K1497007

Naringenin producing operon

This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes:

Figure 2 E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible.

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4-CL <a href="/Part:BBa_K1033001">BBa_K1033001</a>

TAL <a href="/Part:BBa_K1033000">BBa_K1033000</a>

CHI <a href="/Part:BBa_K1497100">BBa_K1497100</a>

CHS <a href="/Part:BBa_K1497101">BBa_K1497101</a>

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For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.

Functional Parameters

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1104
  Illegal NheI site found at 4631
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1671
  Illegal BglII site found at 4640
  Illegal XhoI site found at 3638
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1777
  Illegal NgoMIV site found at 2609
  Illegal NgoMIV site found at 4611
  Illegal NgoMIV site found at 5187
  Illegal AgeI site found at 1872
  Illegal AgeI site found at 2038
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3067
  Illegal BsaI site found at 4560
  Illegal BsaI.rc site found at 1303
  Illegal BsaI.rc site found at 4042