Plasmid_Backbone
pSB1A7
Part:pSB1A7:Design
Designed by: Karmella Haynes Group: iGEM06_Davidson (2006-10-19)
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
Purpose
Read-through transcription is extremely problematic when the usefulness of a device depends upon an off state. This plasmid was constructed to insulate BioBrick parts from read-through transcription coming from the backbone.
Double Forward and Backwards Terminator Assembly
The double terminators were assembled from smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
Source
Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos