Part:BBa_K1405004

McfR

Background

McfR is an TCA intermediates responsive chemoreceptor originally found in Pseudomonas putida. BBa_K1405004 contains McfR coding sequence, an insulator, RBS sequence, and the constitutive promoter BBa_J23100, which is similar to BBa_K515102 that can help us compare our work with iGEM11_Imperial_College_London had done. This device is used as an additional chemoreceptor for endogenous chemotaxis system in E. coli and responds to L(-)malic acid (HO2CCH2CHOHCO2H) and succinate (HOOC-(CH2)2-COOH). A 15 bp insulator sequence upstream of the RBS ensures tunability of expression through easy switching of promoters. In addition it allows the translation initiation rate (TIR) of the RBS to remain the same, when the promoter is replaced. This device is compatible with motile strains of E. coli. It has been transformed and tested in E. coli BL21 in the vector backbone pSB1C3.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Unknown
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1274
  
• 1000
  COMPATIBLE WITH RFC[1000]