Drive the E.coli engineered strain(CL-1) towards death region

Drive the E.coli engineered strain(CL-1)towards death

What it is

Composite part enables the engineered bacteria swim to killing osmotic pressure which will kill bacteria itself. pay attention that this is a truncated version of BBa_K1412010.

How it works

E.coli make use of the EnvZ/OmpR system to mediate signal transduction in response to environmental osmolarity changes. EnvZ, a histidine kinase, undergoes trans-autophosphorylation, then the high energy phosphoryl group is subsequently transferred to OmpR, a response regulator. In the system, OmpR-controlled promoter (PompC) is involved in. The expression strength of PompC is depending upon the medium osmolarity. When medium osmolarity is increasing, the EnvZ will phosphorylate more OmpR into phosphorylated OmpR (OmpR-P), and more OmpR-P will result in stronger expression strength of PompC. In our circuitry design, CheZ is upstream regulated by PompC. As the osmotic pressure is increasing, the motile ability of the engineered E.coli keeps growing, resulting in it's suicide.

What is the difference

Comparing with the biobrick BBa_K1412010, you could find that within BBa_K1412008, the promoter BBa_R0082 is replaced by BBa_R0083. As a result, the length of the device is shortened while the efficiency decreases. (More details about the biobricks BBa_R0082 and BBa_R0083.)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1][http://www.ncbi.nlm.nih.gov/pmc/articles/PMC208463/ Maeda, S. U. M. I. O., and T. A. K. E. S. H. I. Mizuno. "Evidence for multiple OmpR-binding sites in the upstream activation sequence of the ompC promoter in Escherichia coli: a single OmpR-binding site is capable of activating the promoter." Journal of bacteriology 172.1 (1990): 501-503.]