Part:BBa_K1412000

CheZ generator under Lac promoter

---

This part is consist of Lac promoter and CheZ generator.

CheZ can dephosphorylate CheY to make the flagellar motor rotate counterclockwise so that the cell to run and tumble.Without CheZ, CheY is phosphorylated, which can bind to the flagellar motor protein FliM, causing the cell to tumble.so the cell tumbles only. The expression of CheZ allows E.coli to navigate gradients of natural chemoattractants.

At the same time, we know that Lac promoter is an inverting regulator sensitive to LacI and CAP, while LacI can be inhibited by IPTG. So with LacI protein, CAP protein and IPTG,we can adjust the expression of CheZ via Lac promoter to control the movement of E.coli. File:PLac-RBS-CheZ-TT.png

Usage and Biology

---

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Data

Figure 2.

---