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Part:BBa_K1445000:Design
Designed by: Josephina Hendrix Group: iGEM14_CU-Boulder (2014-07-28)
Revision as of 03:21, 11 October 2014 by Johe2273 (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1445002 short</partinfo> <partinfo>BBa_K1445002 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source=== M13 origin of replication from the...")
High copy BioBricked M13 phagemid vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2990
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2996 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2990
Plasmid lacks a suffix.
Illegal XbaI site found at 3005
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1240
Design Notes
Source
M13 origin of replication from the Litmus 28i vector