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Part:BBa_K1445000:Design

Designed by: Josephina Hendrix   Group: iGEM14_CU-Boulder   (2014-07-28)
Revision as of 03:21, 11 October 2014 by Johe2273 (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1445002 short</partinfo> <partinfo>BBa_K1445002 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source=== M13 origin of replication from the...")

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High copy BioBricked M13 phagemid vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2990
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2996
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2990
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3005
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1240


Design Notes

Source

M13 origin of replication from the Litmus 28i vector

References