Composite

Part:BBa_K1412614:Experience

Designed by: Ruihua Zhang   Group: iGEM14_XMU-China   (2014-10-01)
Revision as of 02:51, 11 October 2014 by D (Talk | contribs) (→‎Genetic link stage)

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Protocol

Genetic link stage

1.As a skeleton, TT terminator link with target gene CheZ.

2.Then link the skeleton to target gene CheZ-TT.

3.Next, link RBS-CheZ-TT with promoter Plac.

Genetic link stage

Verification.png

Characterization stage

1.Transfer the part Plac-RBS-CheZ-TT into E.coli (CheZ knocked out), and coat plates, culture for hours to measure the migration diameter of E.coli.

Applications of BBa_K1412614

User Reviews

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