Composite
Part:BBa_K1368000:Design
Designed by: Zhixiang Liu Group: iGEM14_HZAU-China (2014-09-30)
pc1+lox71+TetR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When we built this part, we often failed because of indel in the part.The tetR protein may be poison. so if you want to build other parts like this, you can try to add tetracycline in the LB.
Source
to be finished