Composite

Part:BBa_K1355000

Designed by: Maria Clara Tavares Astolfi, Luna Barroco de Lacerda   Group: iGEM14_UFAM_Brazil   (2014-10-06)
Revision as of 19:58, 10 October 2014 by Lunalacerda (Talk | contribs)

Strong RBS + merA (mercuric ion reductase)+ terminator (BBa_ B0015)

The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system.

LALALAL.jpg


File:MerA protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1200
    Illegal NgoMIV site found at 1262
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
Parameters
None