![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1481005
Designed by: Melania Nowicka Group: iGEM14_PoznanBioInf (2014-10-10)
Strobable rhamnose AND temperature operated transcriptor SR-latch
This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 355
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 125
Illegal BamHI site found at 629
Illegal XhoI site found at 716 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1924
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1944
Illegal BsaI.rc site found at 1463
Illegal BsaI.rc site found at 1827
Illegal BsaI.rc site found at 2101
Illegal SapI.rc site found at 2193
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Categories
Parameters
None |