![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1481003
Designed by: Przemysław Nuc, Melania Nowicka, Weronika Krzynowek, Jakub Bartoszewicz, Katarzyna Rżosińska Group: iGEM14_PoznanBioInf (2014-10-10)
sfGFP with R4-tag under arabinose promoter
This part generates a monomeric red fluorescent protein with his-tag for easy protein purification and R4 degradation tag comprising of four consecutive arginine codons. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for the repression of pBAD in absence of arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1260
[edit]
Categories
Parameters
None |