Part:BBa_K1471005

AGS (Linker) - VP16 (Activation domain).

For this part, we fused the nine base pairs AGS linker subbmited by iGEM13_Freiburg and the Transactivation domain from the VP16 protein from the Herpes Simplex Virus, submitted by iGEM08_ESBS-Strasbourg.

AGS linker is a small sequence of aminoacids (3 aa). Its name is derived from the aminoacids components of its chain, which are Alanine (A), Glycine (G) and Serine (S). It's widely used for fusing proteins domains in Tandem-like synthetic constructs.

VP16 is a transcription factor from Herpes simplex virus that activates the transcription of some viral late genes. In synthetic constructions, VP16 activation domain has probed to be effective at activating the expression of some genes when fused to an adecuate DNA-Binding domain.

This part may be useful to simplify the designing of synthetic constructs intended to be used in eucaryotic cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]