Composite

Part:BBa_K1366102

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)
Revision as of 15:26, 10 October 2014 by Registry (Talk | contribs)

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Genetic construct for msbB gene deletion (C2)

This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 621
    Illegal SapI.rc site found at 831


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Categories
Parameters
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