Composite

Part:BBa_K1431302

Designed by: Rifei Chen, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-10)
Revision as of 09:23, 10 October 2014 by Registry (Talk | contribs)

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SV40 promoter+SV40 PolyA

SV40 promoter is a kind of eukaryocyte cell promoters, of course PolyA also needful. There are two BbsI sites, which recognize six bases[GAAGAC] but cut downstream sites between two bases and four bases, between promoter and PolyA which can make the coding sequence seamless gather with them. So that we can also test how many or what kind of nucleotides interval between promoter and coding sequence will make the highest expression efficiency. For example, if we want to test the efficiency of two nucleotides interval, we should design the the primer sequence like GAAGACNN'NN***, the first NN will be any bases, the second NN is the interval bases,and *** is the begining of coding sequence. If you still do not understand, you can search how BbsI work on the internet or connect with us.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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