Coding

Part:BBa_K1354001

Designed by: Lily Lin, Rebecca Poch, Nolana Wong, Sara Wong, Jae Ho Jang   Group: iGEM14_Cooper_Union   (2014-10-03)
Revision as of 00:01, 10 October 2014 by Rebeccaplease (Talk | contribs)

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VPS75 gene deletion cassette

This sequence is used in Saccharomyces cerevisiae for the knockout of the VPS75 gene with a TRP1 selectable marker. It shortens the initial length of telomeres. In conjunction with an EST2 knockout, the senescence curve of the yeast cells can be compared with those with a single EST2 knockout.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 339
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
    Illegal BamHI site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None