Part:BBa_K1321301

pSEVA321-Bb

This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used it as a shuttle vector for G.xylinus genetic engineering.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3572
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3578
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3572
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3572
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3572
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3587
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 1191
  Illegal NgoMIV site found at 2076
  Illegal NgoMIV site found at 3187
  Illegal NgoMIV site found at 3311
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.