Conjugation

Part:BBa_K1510922

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-09)
Revision as of 17:04, 9 October 2014 by Registry (Talk | contribs)

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Fragrance generator part1: ketoisocaproate decarboxylase (THI3) and RBS

encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.BBa_B0034(RBS)can not only facilitate the transcription rate but also bind to the other two fragrance generators.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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