Coding

Part:BBa_K1539002:Design

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-09)
Revision as of 15:29, 9 October 2014 by Registry (Talk | contribs)

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LPromoter->LRBS->mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS primer construction needed to maintain the EcoRI and XbaI site. The promoter primer design could not maintain the EcoRI site but does contain the XbaI sequence. EcoRI was reestablished when the entire coding sequence was ligated to the backbone.


Source

mCherry from BBa_J06504 RBS and promoter from created primers

References