Coding

Part:BBa_K1539001:Design

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-09)
Revision as of 15:20, 9 October 2014 by Registry (Talk | contribs)

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HPromoter->HRBS->mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS primer construction had to maintain EcoRI and XbaI site. Promoter primer construction had to eliminate the EcoRI site but once ligated to the backbone, this site was reestablished.


Source

mCherry from BBa_J0650 Promoter and RBS originated from primers

References