Composite

Part:BBa_K1363006

Designed by: Bo Dong   Group: iGEM14_USTC-China   (2014-10-09)
Revision as of 08:52, 9 October 2014 by Registry (Talk | contribs)

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an experimental part used to test the conjugation

We add the OriTR to the RFP part,BBa_J04450.Then we transferd it into the Caulobacter crescentus by conjugation.It works in the new bacteria and it confirms that we can transfer other vectors into Caulobacter crescentus by conjugation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal AgeI site found at 1160
    Illegal AgeI site found at 1272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19


[edit]
Categories
Parameters
n/aan experimental part used to test the conjugation