Deleted
Sample Discontinued
Experience: Works
Not Used
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Plasmid

Part:BBa_K1321202

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 08:36, 9 October 2014 by Registry (Talk | contribs)

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VHb in pSEVA321-Bb

VhB in pSEVA-321

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4084
    Illegal suffix found in sequence at 513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4084
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 514
    Illegal PstI site found at 528
    Illegal NotI site found at 521
    Illegal NotI site found at 4090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4084
    Illegal BglII site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4084
    Illegal suffix found in sequence at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4084
    Illegal XbaI site found at 4099
    Illegal SpeI site found at 514
    Illegal PstI site found at 528
    Illegal NgoMIV site found at 1703
    Illegal NgoMIV site found at 2588
    Illegal NgoMIV site found at 3699
    Illegal NgoMIV site found at 3823
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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