Composite

Part:BBa_K1326003

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)
Revision as of 02:48, 9 October 2014 by Registry (Talk | contribs)

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Plac + POR + DVR1 + ChlP + ChlG

Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 808
    Illegal BglII site found at 4090
    Illegal BglII site found at 4579
    Illegal BamHI site found at 1571
    Illegal BamHI site found at 3859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 347
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2461


[edit]
Categories
Parameters
None