Composite
Part:BBa_K1326003
Designed by: Leah Simmons Group: iGEM14_Macquarie_Australia (2014-10-08)
Plac + POR + DVR1 + ChlP + ChlG
Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 808
Illegal BglII site found at 4090
Illegal BglII site found at 4579
Illegal BamHI site found at 1571
Illegal BamHI site found at 3859 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 347
Illegal AgeI site found at 398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2461
[edit]
Categories
Parameters
None |