Coding

Part:BBa_J52011

Designed by: Monika Ciglic   Group: iGEM06_Slovenia   (2006-10-14)
Revision as of 08:07, 14 October 2006 by Registry (Talk | contribs)

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dnMyD88-linker-Rluc

dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 982


[edit]
Categories
//cds/enzyme
//chassis/eukaryote/human
//function/immunology
//function/reporter/light
Parameters
None