Reporter

Part:BBa_J33203

Designed by: Chris French   Group: iGEM06_Edinburgh   (2006-10-13)
Revision as of 16:31, 13 October 2006 by Registry (Talk | contribs)

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E. coli ars promoter, arsR gene and lacZ

This biobrick contains the promoter region of the Escherichia coli chromosomal arsenic detoxification opern, including the arsR gene encoding the ArsR repressor protein required for inducibility of this promoter. This is fused to a lacZ' minigene encoding the N-terminal 77 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation. When introduced into an E. coli host strain carrying the lacZ-delta-M15 mutation, this construct results in lacZ expression in the presence of arsenate or arsenite anion. LacZ expression can be detected in various ways. This construct was designed for the detection of arsenate and arsenite in water samples.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None