Generator

Part:BBa_J3107:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-10-12)
Revision as of 21:06, 12 October 2006 by Kahaynes (Talk | contribs) (→‎Source)

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AraC-PC, pLac-Hin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

A double forward terminator is placed upstream of "pLac-Hin" to block forward read-through transcription from the pSB1AK3 vector. This should allow tighter control over Hin expression.


Source

AraC-PC-TT - part BBa_S03591, a composite of BBa_I13458 and part BBa_B0015

pLac-RBS-Hin-TT - part BBa_S03537

References