Coding
ComX

Part:BBa_K1379004:Design

Designed by: Jordy Evan, Nadia Benedicta, Angeline Widjaja   Group: iGEM14_Hong_Kong_HKUST   (2014-09-23)
Revision as of 15:42, 8 October 2014 by Ngsiuwang (Talk | contribs)

S. pneumoniae σx CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 298


Design Notes

None

Source

iGEM 2014 Hong_Kong_HKUST Team has cloned σX from S. pneumoniae strain NCTC7465 by PCR, followed by restriction-ligation into pSB1C3 backbone.

References

Luo Ping, & Morrison Donald. (2003). Transient Association of an Alternative Sigma Factor, ComX, with RNA Polymerase during the Period of Competence for Genetic Transformation in Streptococcus pneumoniae. Journal of Bacteriology. doi:10.1128/JB.185.1.349-358.2003
Piotrowski Andrew, Luo Ping, & Morrison Donald. (2009). Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW. doi:10.1128/JB.01750-08