Generator
ComX

Part:BBa_K1379006:Design

Designed by: Jordy Evan, Nadia Benedicta, Angeline Widjaja   Group: iGEM14_Hong_Kong_HKUST   (2014-09-23)
Revision as of 15:24, 8 October 2014 by Hyht2011 (Talk | contribs) (→‎Design Notes)


S. pneumoniae σx Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359


Design Notes

This σX Generator was constructed by restricting-ligating to downstream of [Part:BBa_K880005|BBa_K880005] first and then subsequently to [Part:BBa_B0015|BBa_B0015] in [Part:pSB1C3|pSB1C3].

Source

iGEM 2014 Hong_Kong_HKUST Team has cloned σX from S. pneumoniae strain NCTC7465.

References