Tag
His6
Part:BBa_K1362431:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-08)
N-terminal His6 (C-Intein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Golden Gate insert with RFC[???] standard overhangs. Can therefore be used to add an His6 to any intein in a standard construct. See further information on the iGEM team Heidelberg 2014's wiki or in the RFC specifications [1].
Source
from wikipedia