Device

Part:BBa_K1465227:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-07)
Revision as of 02:20, 8 October 2014 by Tore (Talk | contribs) (→‎Design Notes)

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sRNA of phosphofructokinase A under the control of T7 promoter (T7_sRNA:pfkA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 135
    Illegal XhoI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA containing the anti-sense RNA for the phosphofructokinase (pfkA) of Escherichia coli (BBa_K1465225) into the pSB1C3 backbone of the T7 promotor (BBa_I719005).

Source

BBa_K1465225: sRNA:pfkA.
BBa_I719005: T7 promoter

References