Part:BBa_K1317003:Experience

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Applications of BBa_K1317003

The protein ELP has been produced in BL21 strain of E.coli. After phase cycling purification it is possible to spin the protein into a wire, thanks to the wet-spinning method. The ELP can have different length regarding the number of copy of the gene. In these experiments, we used 40mer ELP (assemble of two gene copies).

Polymer formation trial:

In order to obtain polymer wires, we carried out severeal tests by mixing the ELP with different solvants. The following results have been observed:

- Sodium Sulfate (Na2So4) at 320 g/L => cottony aspect of the fiber, the structure is not stable

- Diethyl ether (C2H5)2O => No reaction because the drop is trapped. This solvent is not miscible with the polymer

- Acetone (CH3COCH3) => Formation of a white ring

- Heated Sodium Chloride (NaCl 5M) => Formation of a white cloud due to a lack of cohesiveness

- Calcium Chloride (CaCl2 15%) => 1.25% alginate mixture with ELP40 at 10mg/mL: a white-colored fiber is obtained This last trial has been performed with 0.75% alginate which seems to be the best experimental condition to get fibers from the ELP

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