Device

Part:BBa_K1329003

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-07)
Revision as of 17:01, 7 October 2014 by Registry (Talk | contribs)

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Hag-D2-Strep

In order to build up flagella usable as protein scaffold we wanted to design a system which makes it possible to switch a high variety of functional modules. For that purpose we designed a flagellin construct containing a Strep-Tag inserted into the D2 domain of Salmonella typhimurium on top of the Bacillus Subtilis flagellin as a linker region. By exposure of the Strep-Tag to the outside of the flagellum it would be possible to connect Streptavidin-hybridized fusion proteins of various functionalities to the flagellum as scaffold increasing the epitope density.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


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Categories
Parameters
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