Device

Part:BBa_K1329002

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-07)
Revision as of 16:24, 7 October 2014 by Registry (Talk | contribs)

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Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin

The bacterial flagellum is built up of multiple copies of a protein called flagellin. The structure of this protein is mainly conserved between different species, but the variation of a loop directed to the outside of the flagellum can be observed. As we aim to fish metal ions from solution the bacterial flagellum was chosen as a protein scaffold system. We inserted the metallothionein Cup1-1 from Saccharomyces cerevisiae into the variable loop of B. subtilis flagellin. Cup1-1 can bind up to 7 ions of either copper or silver.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


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