Coding

Part:BBa_K1317002:Design

Designed by: Mounir Benkoulouche   Group: iGEM14_Bordeaux   (2014-10-07)
Revision as of 14:44, 7 October 2014 by Mounir (Talk | contribs) (→‎Source)


CDS of silk-like protein (SLP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.


Source

major ampullate spidroin 1 (MaSp1) gene from Nephila clavipes

References