Reporter

Part:BBa_K1497022

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 13:59, 7 October 2014 by Registry (Talk | contribs)

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Naringenin sensor (FdeR) with CFP as reporter

We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 95
    Illegal NgoMIV site found at 452
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 271


[edit]
Categories
//cds/reporter
//cds/reporter/cfp
//function/sensor
Parameters
n/aNaringenin sensor (FdeR) with CFP as reporter