Generator

Part:BBa_K1497017

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 13:28, 7 October 2014 by Registry (Talk | contribs)

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Naringenin producing operon under the T7 promoter BBa_I712074

Transformed into BL21(DE3) and induced with IPTG this composite is able to produce naringenin from tyrosine. It consists of 4 genes:

4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497000) CHS (BBa_K1497001)

For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.

For characterization the naringenin biosensor K1497020 was used. After 24 h incubation GFP fluorescence was visible to the unaided eye. We measured the relative GFP fluorescence by use of OptimaFluoroStar.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1158
    Illegal NheI site found at 4685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1725
    Illegal BglII site found at 4694
    Illegal XhoI site found at 3692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2663
    Illegal NgoMIV site found at 4665
    Illegal NgoMIV site found at 5241
    Illegal AgeI site found at 1926
    Illegal AgeI site found at 2092
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3121
    Illegal BsaI site found at 4614
    Illegal BsaI.rc site found at 1357
    Illegal BsaI.rc site found at 4096


[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
n/aNaringenin producing operon under the T7 promotor BBa_I712074