Generator
Part:BBa_K1497017
Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen Group: iGEM14_TU_Darmstadt (2014-10-07)
Naringenin producing operon under the T7 promoter BBa_I712074
Transformed into BL21(DE3) and induced with IPTG this composite is able to produce naringenin from tyrosine. It consists of 4 genes:
4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497000) CHS (BBa_K1497001)
For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.
For characterization the naringenin biosensor K1497020 was used. After 24 h incubation GFP fluorescence was visible to the unaided eye. We measured the relative GFP fluorescence by use of OptimaFluoroStar.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1158
Illegal NheI site found at 4685 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1725
Illegal BglII site found at 4694
Illegal XhoI site found at 3692 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1831
Illegal NgoMIV site found at 2663
Illegal NgoMIV site found at 4665
Illegal NgoMIV site found at 5241
Illegal AgeI site found at 1926
Illegal AgeI site found at 2092 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3121
Illegal BsaI site found at 4614
Illegal BsaI.rc site found at 1357
Illegal BsaI.rc site found at 4096
[edit]
Categories
Parameters
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
//cds/biosynthesis/anthocyanins
n/a | Naringenin producing operon under the T7 promotor BBa_I712074 |