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Regulatory

Part:BBa_K1351024

Designed by: Judith Heckmann   Group: iGEM14_LMU-Munich   (2014-10-06)
Revision as of 12:18, 7 October 2014 by JudithHeckmann (Talk | contribs)

PcomC, a CSP inducible promoter derived from Streptococcus pneumoniae

CSP-depending promoter of Streptococcus pneumoniae (R6). This regulatory sites have been shown to be sufficient to drive ComE regulated transcription within S. pneumoniae[http://www.ncbi.nlm.nih.gov/pubmed/?term=comE%2FcomE~P+interplay].

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//regulation
Parameters
None