Composite

Part:BBa_K1497007

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 12:06, 7 October 2014 by Registry (Talk | contribs)

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Naringenin producing operon

This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes:

4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497100) CHS (Bba_K1497101)

For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
    Illegal NheI site found at 4631
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1671
    Illegal BglII site found at 4640
    Illegal XhoI site found at 3638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1777
    Illegal NgoMIV site found at 2609
    Illegal NgoMIV site found at 4611
    Illegal NgoMIV site found at 5187
    Illegal AgeI site found at 1872
    Illegal AgeI site found at 2038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3067
    Illegal BsaI site found at 4560
    Illegal BsaI.rc site found at 1303
    Illegal BsaI.rc site found at 4042


[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
None