Translational_Unit

Part:BBa_K1497004

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 11:17, 7 October 2014 by Registry (Talk | contribs)

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B0034-CHS - Chalcone Synthase from Gerbera with strong RBS

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 1099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472


[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
n/aB0034-CHS - Chalcone Synthase from Gerbera with strong RBS