Composite

Part:BBa_K1341000

Designed by: Run-ze Zhang   Group: iGEM14_AHUT_China   (2014-09-27)
Revision as of 01:40, 7 October 2014 by Zrz578805758 (Talk | contribs)

Example.jpg switch device(LTT gfp)for our bio-compass

We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.

Assembly standards

This part is based on Part BBa_K325219. We resynthesised this part to remove three PstI restriction sites, making it compatible with BioBrick assembly standards 10 and 23.

Unfortunately, in the process of mutagenesis to create the colour change a SpeI site was introduced at site 3044. We have ordered primers to remove this site by a substitution from T->C at base 3046. BBa K325259 (planning) will be the successor to this part compatible with Assembly Standards 10 and 23. Unfortunately we did not have time to complete this before the submission deadline, therefore the part will be submitted during November 2010. In the meantime it is possible to use this part with RFC 57.

Functional Parameters

Functional Parameters

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 732


Functional Parameters


Sequences


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334
[edit]
Categories
Parameters
None