Composite
Part:BBa_K1336006:Design
Designed by: Yan-Kay Ho Group: iGEM14_UCL (2014-10-06)
LacI Expression Cassette with ispB RC gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).
Source
From genomic sequence of Escherichia coli.