Composite

Part:BBa_K1336006:Design

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)
Revision as of 22:20, 6 October 2014 by Registry (Talk | contribs)

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LacI Expression Cassette with ispB RC gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).


Source

From genomic sequence of Escherichia coli.

References