Coding

Part:BBa_K1448000

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-09-22)
Revision as of 21:54, 6 October 2014 by UMBABA (Talk | contribs) (→‎Picture of our designned part)

gamma-terpinene synthase

gamma-terpinene synthase produces gamma-terpinene from GPP,geranyl diphosphate.

This part contains gamma-terpinene synthase region of Citrus unshiu and was isolated by Mr.Shimada from Citrus unshiu.http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003


Background and prnciples

Characterization

Gel picture of our designned part

Westernbloting analysis

Fig1,Western blot analysis of GST and d-limonene synthase(Lane2), ɤ-terpinene synthase(Lane3) and β-pinene synthases(Lane4) fusion proteins expressed by yeast. Lanes: 1, p427-TEF; 2, p427-TEF-LS; 3, p427-TEF-gTS; 4, p427-TEF-PS.

Reference


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 204
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/agamma-terpinene synthase