Composite
pBAD-BtGal

Part:BBa_K1489000:Design

Designed by: Iowis Zhu   Group: iGEM14_UMaryland   (2014-10-06)
Revision as of 20:44, 6 October 2014 by HHeLiBeBCNOFNe (Talk | contribs) (→‎References)


pBAD-RBS-Bovine Galectin-1-B0012 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 499
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EcoRI site in middle of Bovine Galectin gene was removed, codons were optimized for expression in E. coli.


Source

Sequence generated from Bos taurus genome, reoptimized for E. coli expression.

References

Bourne Y, Bolgiano B, Liao DI, et al. Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol. 1994;1(12):863-70.