Regulatory

Part:BBa_K1400003

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)
Revision as of 19:52, 6 October 2014 by Dylansiriwardena (Talk | contribs)

pTre(4) Single input tet responsive promoter

A single tet responsive promoter created by removing the 2 most distal (from TATA box) gal4 sites, and replacing the last 2 gal4 sites in the gal promoter with tet sites. THe two mig1 sites were replaced as well. It was designed to have similar expression to our modified gal promoter (BBA_K1400002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


[edit]
Categories
Parameters
None