Coding
mRdRP

Part:BBa_K1442101

Designed by: Dan Goss   Group: iGEM14_Warwick   (2014-10-06)
Revision as of 16:06, 6 October 2014 by Dangoss (Talk | contribs)

RdRP Mutant

To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga). Therefore, the region beyond said codon remains untranslated, so the phenotype of this mutant is determined entirely by the region previous to the stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None